Library Construction Service

Library Construction Service
Service Introduction

AlpalifeBio has rich experiences in the construction of nanobody libraries, and adopts optimized primers to ensure diverse high-quality phage display libraries.

Phage Display Library Generation

Construct diverse nanobody/VHH DNA sequences in phagemids, transform into TG1 competent cells, culture and induce VHH display on phage surface.

Yeast Library Construction

Insert diverse nanobody/VHH  DNA sequences into the display plasmid vector containing the protein scaffold-Aga2 gene, and transform the competent yeast to prepare the yeast display library.
Technical Advantages

Extracted RNA with high quality lays a solid foundation for library construction Optimized primers can ensure VHH library diversity.
Optimized competent preparation method can ensure high conversion rate.

Service Content

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